Polskie Towarzystwo
Hematologów i Transfuzjologów

czcionka: [+] powiększ | [-] pomniejsz

Acta Haematologica Polonica, 2010, 41, 4

ANNA PITUCH-NOWOROLSKA · JERZY KOWALCZYK AND POLISH GROUP FOR ACUTE LEUKAEMIA AND NON-HODGKIN’S LYMPHOMA DIAGNOSIS AND THERAPY IN CHILDREN

The frequency and characteristics of aberrant immunophenotypes in acute lymphoblastic leukaemia (ALL) in children – a multicenter study

Częstość występowania i charakterystyka nietypowych immunofenotypów w ostrych białaczkach limfoblastycznych u dzieci – analiza wieloośrodkowa

SłOWA KLUCZOWE:
Ostra białaczka limfoblastycznaDzieciNietypowe immunofenotypyWielokolorowa cytometria
KEY WORDS:
Acute lymphoblastic leukaemiaChildrenAtypic immunophenotypesMulticolour cytometry
pełna treść »
STRESZCZENIE: The immunophenotype of acute lymphoblastic leukaemia (ALL) cells is used for classification of leukaemia based on origin and maturation/differentiation stage of leukaemia cells. The aberrant expression of lineage restricted and line associated determinants on the surface and in cytoplasm of leukaemia cells (atypic immunophenotypes) is useful for detection of minimal residual disease (MRD) by flow cytometry. The multicenter studies of typical and atypical immunophenotypes of leukaemia cells enrolled 645 children with diagnosis of B cell origin (86.54%) and T cell origin (13.46%) ALL. Atypical immunophenotypes were noted in 204 cases (31.62%) with myeloid co-expression (CD13 and/or CD33, CD15) as the most frequent type of aberrant determinant expression. In B cell line-originating ALL, the frequency of atypical immunophenotypes decreased along with the maturation of B cells (ALL progenitor B – 46.65%, common ALL – 32.6%, ALL-pre B and ALL-B – 17.8%). In the group of T-ALL, atypical immunophenotypes were noted in 22 cases (25.2%). The analysis of aberrant expression of determinants showed an association between CD34 and myeloid co-expression (CD33, CD13) and a reverse association between CD34 and CD20.
The percentage and character of atypical immunophenotypes observed in ALL patients indicate the effectiveness of MRD detection by flow cytometry in above 60% of ALL cases in children.
SUMMARY: The immunophenotype of acute lymphoblastic leukaemia (ALL) cells is used for classification of leukaemia based on origin and maturation/differentiation stage of leukaemia cells. The aberrant expression of lineage restricted and line associated determinants on the surface and in cytoplasm of leukaemia cells (atypic immunophenotypes) is useful for detection of minimal residual disease (MRD) by flow cytometry. The multicenter studies of typical and atypical immunophenotypes of leukaemia cells enrolled 645 children with diagnosis of B cell origin (86.54%) and T cell origin (13.46%) ALL. Atypical immunophenotypes were noted in 204 cases (31.62%) with myeloid co-expression (CD13 and/or CD33, CD15) as the most frequent type of aberrant determinant expression. In B cell line-originating ALL, the frequency of atypical immunophenotypes decreased along with the maturation of B cells (ALL progenitor B – 46.65%, common ALL – 32.6%, ALL-pre B and ALL-B – 17.8%). In the group of T-ALL, atypical immunophenotypes were noted in 22 cases (25.2%). The analysis of aberrant expression of determinants showed an association between CD34 and myeloid co-expression (CD33, CD13) and a reverse association between CD34 and CD20.
The percentage and character of atypical immunophenotypes observed in ALL patients indicate the effectiveness of MRD detection by flow cytometry in above 60% of ALL cases in children.

pełna treść:

pobierzpobierz pełną treść publikacji (PDF 188kb)

Pobierz program Adobe Reader®Do otwarcia plików PDF niezbedny jest darmowy program Adobe Reader® Jeśli nie masz tego programu pobierz go tutaj